1113
1113 .
LEPTOSPIRA-MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Leptospira idonii DSM 26084
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1113 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Leptospira Medium Base EMJH
(Difco) |
2.3 | g | 2.3 | - | |
| Agarose | 1.5 | g | 1.5 | 2.379 | |
| Water | 900.0 | ml | - | - | |
| Leptospira Enrichment EMJH | 100.0 | ml | - | - | |
| 1 Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C. | |||||
Metadata
| Last modified: | 01.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 15 |
| Strains with modifications: | 10 |
Leptospira idonii DSM 26084
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Leptospira Medium Base EMJH | 2.3 | - |
| Agarose | 1.5 | 2.379 |
| Water | - | - |
| Leptospira Enrichment EMJH | - | - |
Cultivation metadata from 15 strains
| phyla | 2 | 50 strains |
| oxygen | aerobe | 3 strains |
| oxygen | anaerobe | 1 strain |
| temperature | mesophilic | 15 strains |
| temperature | psychrophilic | 1 strain |