1113 .
LEPTOSPIRA-MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Leptospira Medium Base EMJH
(Difco) |
2.3 | g | ||
|
|
Agarose | 1.5 | g | ||
|
|
Water | 900.0 | ml | ||
|
|
Leptospira Enrichment EMJH | 100.0 | ml | ||
| 1 Dissolve the agarose in water by boiling. Add the Leptospira base and dissolve completely. Autoclave at 121°C for 15 min. Allow the medium to cool to 50-55°C. Warm the Leptospira Enrichment to approximately 25°C and add to the warm medium. Mix uniformly and dispense aseptically into sterile tubes as requested. Final pH is 7.5 at 25°C. | |||||