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DSMZ

DSMZ 1115 . CONGREGIBACTER (SYPHC) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1115 #

ml
Equipment needed: Filter Gassing station Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA) 		35.00 g 34.965 -
Wolfe's mineral elixir 1.00 ml - -
NH4Cl 0.10 g 0.1 1.868
Yeast extract 1.00 g 0.999 -
Na-pyruvate 1.10 g 1.099 9.986
HEPES
(SIGMA) 		2.50 g 2.498 10.481
L-Histidine 0.04 g 0.04 0.258
L-Cysteine HCl x H2O 0.04 g 0.04 0.228
Seven vitamins solution 0.50 ml - -
KH2PO4 0.05 g 0.05 0.367
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except vitamins and hydrogenphosphate) and adjust pH to 7.7. After autoclaving add vitamins and hydrogenphosphate from sterile stock solutions sterilized by filtration. Adjust pH of complete medium to 7.3 - 7.5, if necessary.
2 Note: Growth of most strains is stimulated by using microaerophilic conditions with only 10% (v/v) oxygen in the head space gas atmosphere. For microaerophilic growth medium is prepared without HEPES, sparged with 80% N2 and 20% CO2 gas mixture to make it anoxic and then dispensed under air atmosphere into serum vials to 50% volume. After autoclaving the pH is adjusted to 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture.

Wolfe's mineral elixir #

1000
Solution occurs in 21 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3 - 7.5
Equipment needed: Autoclave, Filter, Gassing station
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.965 -
HEPES 2.498 10.481
Na-pyruvate 1.099 9.986
Yeast extract 0.999 -
NH4Cl 0.1 1.868
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
oxygen microaerophile 2 strains
temperature mesophilic 3 strains