1118
1118 .
MD1-MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1118 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casitone | 3.0 | g | 3 | - | |
| CaCl2 x 2 H2O | 0.7 | g | 0.7 | 4.761 | |
| MgSO4 x 7 H2O | 2.0 | g | 2 | 8.114 | |
| Trace element solution SL-4 | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| Vitamin B12 | 0.5 | mg | 5.0e-4 | 3.7e-4 | |
| 1 After autoclaving and cooling, add 0.5 mg /l vitamin B12 from a filter sterilized stock solution. pH should be 7.0 -7.2. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 0.50 | g | 0.5 | 1.711 |
| FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 |
| ZnSO4 x 7 H2O | 0.01 | g | 0.01 | 0.035 |
| MnCl2 x 4 H2O | 3.00 | mg | 0.003 | 0.015 |
| H3BO3 | 0.03 | g | 0.03 | 0.485 |
| CoCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| CuCl2 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.006 |
| NiCl2 x 6 H2O | 2.00 | mg | 0.002 | 0.015 |
| Na2MoO4 x 2 H2O | 3.00 | mg | 0.003 | 0.012 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 04.06.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casitone | 3 | - |
| MgSO4 x 7 H2O | 2 | 8.114 |
| CaCl2 x 2 H2O | 0.7 | 4.761 |
| EDTA | 5.0e-4 | 0.002 |
| Vitamin B12 | 5.0e-4 | 3.7e-4 |
| Show more | ||
