1119
1119 .
GNYS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Acidisphaera rubrifaciens DSM 16009
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 1119 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sodium gluconate | 3.00 | g | 3 | 13.753 | |
|
Yeast extract
(Difco) |
1.00 | g | 1 | - | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| NaCl | 0.20 | g | 0.2 | 3.422 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Trace element solution SL8 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , for solid medium | 1.80 | % | 18 | 53.519 | |
| 1 Adjust pH to 4.0 - 4.5. | |||||
| 2 For solid medium, add 1.8% agar (final) and adjust pH to 5.0 to avoid hydrolysis of agar. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 5.2 | g | 5.2 | 15.467 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 |
| H3BO3 | 62.0 | mg | 0.062 | 1.003 |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 4.0 - 4.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Acidisphaera rubrifaciens DSM 16009
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 18 | 53.519 |
| Sodium gluconate | 3 | 13.753 |
| (NH4)2SO4 | 1 | 7.568 |
| KH2PO4 | 1 | 7.348 |
| Yeast extract | 1 | - |
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