Culture medium recipe
Vol.
×
Main sol. 113 #
ml
Equipment needed: Gassing station
Autoclave
Filter
Hungate tubes
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 960 | ml | - | - | |
Solution B | 20 | ml | - | - | |
Solution C | 20 | ml | - | - | |
Solution D | 1 | ml | - | - | |
1 Solutions A, B and D are sterilized separately by autoclaving at 121°C for 15 minunder 100% N2 gas atmosphere. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. | |||||
2 Note: A small amount of white precipitate forms after autoclaving, but this has no negative effect on growth. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 2.0 | g | 2.083 | 15.309 | |
KNO3 | 2.0 | g | 2.083 | 20.606 | |
NH4Cl | 1.0 | g | 1.042 | 19.474 | |
MgSO4 x 7 H2O | 0.8 | g | 0.833 | 3.381 | |
Trace element solution SL-4 | 2.0 | ml | - | - | |
Agar , for solid medium | 15.0 | g | 15.625 | 46.457 | |
Distilled water | 960.0 | ml | - | - | |
1 Adjust pH to 7.0 with NaOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2S2O3 x 5 H2O | 5 | g | 250 | 1007.31 |
Distilled water | 20 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaHCO3 | 1 | g | 50 | 595.188 |
Distilled water | 20 | ml | - | - |
Equipment needed: Filter
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FeSO4 x 7 H2O | 2 | mg | 2 | 7.194 |
H2SO4
(0.1 N) |
1 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
No comments for this medium.