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DSMZ 113 . THIOBACILLUS DENITRIFICANS MEDIUM

Culture medium recipe

Vol.
×

Main sol. 113 #

ml
Equipment needed: Gassing station Autoclave Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 960 ml - -
Solution B 20 ml - -
Solution C 20 ml - -
Solution D 1 ml - -
1 Solutions A, B and D are sterilized separately by autoclaving at 121°C for 15 minunder 100% N2 gas atmosphere. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated.
2 Note: A small amount of white precipitate forms after autoclaving, but this has no negative effect on growth.

Solution A #

960
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.0 g 2.083 15.309
KNO3 2.0 g 2.083 20.606
NH4Cl 1.0 g 1.042 19.474
MgSO4 x 7 H2O 0.8 g 0.833 3.381
Trace element solution SL-4 2.0 ml - -
Agar , for solid medium 15.0 g 15.625 46.457
Distilled water 960.0 ml - -
1 Adjust pH to 7.0 with NaOH.

Solution B #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 250 1007.31
Distilled water 20 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 50 595.188
Distilled water 20 ml - -

Solution D #

1
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 2 mg 2 7.194
H2SO4
(0.1 N) 		1 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes

Gas composition

  • 100 % N2

Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2S2O3 x 5 H2O 5 20.146
KNO3 2 19.782
KH2PO4 2 14.697
NH4Cl 1 18.695
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Cultivation metadata from 5 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 5 strains
oxygen facultative anaerobe 3 strains
oxygen anaerobe 2 strains
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