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1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Modified MJ synthetic sea water 1000 ml - -
2 1 g 1 11.765
3 1 g 1 11.904
4
(powder)
3 g 3 93.557
Instructions:
  1. Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.703 508.243
2 0.14 g 0.139 0.796
3 0.50 g 0.495 3.367
4 0.50 g 0.495 9.255
5 3.40 g 3.366 13.658
6 4.18 g 4.139 20.357
7 0.33 g 0.327 4.383
8 0.01 g 0.01 0.036
9 1.00 mg 9.9e-4 0.004
10 1.00 mg 9.9e-4 0.004
11 Trace element solution 10.00 ml - -
12 1000.00 ml 988.324 54860.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 0.356
7 0.10 g 0.1 0.68
8 0.10 g 0.1 0.348
9 0.01 g 0.01 0.04
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
Solution occurs in 12 other media (first defined in medium 1668 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.013
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Instructions:
  1. Filter sterilize and make anoxic with N2.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 29.713 508.412
MgCl2 x 6 H2O 4.139 20.357
MgSO4 x 7 H2O 3.396 13.778
Sulfur 3 93.557
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Cultivation metadata from 2 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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