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1132. LC 2

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.995 4.037
2 0.20 g 0.199 1.354
3 0.05 g 0.05 0.492
4 Trace element solution SL-10 1.00 ml - -
5 1000.00 ml 993.241 55133.2
6 Phosphate buffer
(pH 6.8, see below)
2.00 ml - -
7 1.00 ml - -
8
(4mg/ml)
1.00 ml - -
9 0.30 ml 0.236 7.36
10 Vitamin solution , might need strain adjustment 1.00 ml - -
Instructions:
  1. Dispense 5 ml aliquots into Hungate tubes. After autoclaving add the following components per liter: #6-9
  2. Incubate in sealed containers. The strain held in the DSMZ has been cultivated on methanol, but the original publication indicates that the strain will also grow on methane.
Solution occurs in 32 other media (first defined in medium 461 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%; 7.7 M)
10.0 ml 3.17 86.943
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.6 g 36 253.594
2 1.4 g 14 102.877
3 100.0 ml 998.207 55408.8
Instructions:
  1. Adjust to pH 6.8.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.211 55298
MgSO4 x 7 H2O 0.995 4.037
Methanol 0.236 7.36
CaCl2 x 2 H2O 0.199 1.354
Na2HPO4 0.072 0.505
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Cultivation metadata from 2 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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