Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
CaCl2 x 2 H2O | 0.20 | g | 0.2 | 1.36 | |
KNO3 | 0.05 | g | 0.05 | 0.495 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
H2O | 1000.00 | ml | - | - | |
1 Dispense 5 ml aliquots into Hungate tubes. After autoclaving add the following components per liter: | |||||
Phosphate buffer
(pH 6.8) |
2.00 | ml | - | - | |
HEPES buffer
(pH 7.0, 2 M) |
1.00 | ml | 0.477 | 2 | |
Fe(III)NH4-EDTA
(4mg/ml) |
1.00 | ml | - | - | |
Methanol | 0.30 | ml | 0.238 | 7.415 | |
2 Adjust to pH 6.8. | |||||
3 Incubate in sealed containers. The strain held in the DSMZ has been cultivated on methanol, but the original publication indicates that the strain will also grow on methane. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.8 |
Equipment needed: | Hungate tubes |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgSO4 x 7 H2O | 1 | 4.057 |
HEPES buffer | 0.477 | 2 |
Methanol | 0.238 | 7.415 |
CaCl2 x 2 H2O | 0.2 | 1.36 |
Na2HPO4 | 0.072 | 0.507 |
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