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DSMZ

DSMZ 1136 . RHODOVULUM KHOLENSE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1136 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
CaCl2 x 2 H2O 0.12 g 0.12 0.816
MgSO4 x 7 H2O 2.00 g 2 8.114
NH4Cl 0.64 g 0.64 11.965
NaCl 20.00 g 20 342.231
Yeast extract 0.50 g 0.5 -
Sodium pyruvate 3.00 g 3 27.263
Trace element solution SL-12 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust to pH 7.0
2 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.

Trace element solution SL-12 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.000 g 3 8.923
FeSO4 x 7 H2O 1.100 g 1.1 3.957
ZnCl2 0.042 g 0.042 0.308
MnCl2 x 4 H2O 0.050 g 0.05 0.253
H3BO3 0.300 g 0.3 4.851
CoCl2 x 6 H2O 0.190 g 0.19 1.463
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
NiCl2 x 6 H2O 0.024 g 0.024 0.185
Na2MoO4 x 2 H2O 0.018 g 0.018 0.074
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Sodium pyruvate 3 27.263
MgSO4 x 7 H2O 2 8.114
NH4Cl 0.64 11.965
KH2PO4 0.5 3.674
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Cultivation metadata from 0 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
oxygen facultative anaerobe 1 strain
oxygen obligate anaerobe 1 strain
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