113 .
THIOBACILLUS DENITRIFICANS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 962 | ml | ||
|
|
Solution B | 20 | ml | ||
|
|
Solution C | 20 | ml | ||
|
|
Solution D | 1 | ml | ||
| 1 Solutions A, B, and D are sterilized separately by autoclaving at 121°C for 15 min under a 100% N2 gas atmosphere. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust it to 7.0 - 7.2, if necessary. | |||||
Solution A #
962
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
KH2PO4 | 2.0 | g |
|
|
KNO3 | 2.0 | g |
|
|
NH4Cl | 1.0 | g |
|
|
MgSO4 x 7 H2O | 0.8 | g |
|
|
Trace element solution SL-4 | 2.0 | ml |
|
|
Distilled water | 960.0 | ml |
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||