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DSMZ

DSMZ 113a . SULFURIMONAS HONGKONGENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 113a #

ml
Equipment needed: Gassing station Autoclave Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 942 ml - -
Solution B 40 ml - -
Solution C 20 ml - -
Solution D 1 ml - -
Solution E 1 ml - -
1 Solution A is prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by autoclaving under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solutions B, D, and E are prepared under a 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust to pH 7.0-7.2, if necessary.

Solution A #

942
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.0 g 26.539 454.129
KH2PO4 2.0 g 2.123 15.602
KNO3 2.0 g 2.123 21
NH4Cl 1.0 g 1.062 19.846
MgSO4 x 7 H2O 0.8 g 0.849 3.446
Trace element solution SL-4 2.0 ml - -
Distilled water 940.0 ml - -

Solution B #

40
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 125 503.655
Distilled water 40 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 20 mg 2 7.194
H2SO4
(0.1 N) 		10 ml - -

Solution E #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 17.09.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.9 426.085
Na2S2O3 x 5 H2O 4.98 20.066
KNO3 1.992 19.703
KH2PO4 1.992 14.638
Na2CO3 0.996 9.397
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain