DSMZ 113a . SULFURIMONAS HONGKONGENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Autoclave Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 930 ml - -
Solution B 40 ml - -
Solution C 20 ml - -
Solution D 1 ml - -
Solution E 10 ml - -
1 Solutions A, B and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121°C for 15 min. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. For solid medium add 15.0 g/l agar to solution A.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.0 g 26.882 459.988
KH2PO4 2.0 g 2.151 15.803
KNO3 2.0 g 2.151 21.271
NH4Cl 1.0 g 1.075 20.102
MgSO4 x 7 H2O 0.8 g 0.86 3.49
Trace element solution SL-4 2.0 ml - -
Agar , for solid medium
optional
15.0 g/l 15 44.599
Distilled water 930.0 ml - -
1 Adjust pH to 7.0 with NaOH.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 125 503.655
Distilled water 40 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 50 595.188
Distilled water 20 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 2 mg 2 7.194
H2SO4
(0.1 N)
1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Agar
optional
13.95 41.477
Na2S2O3 x 5 H2O 5 20.146
KNO3 2 19.782
KH2PO4 2 14.697
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain