Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Solution A | 942 | ml | ||
|
Solution B | 40 | ml | ||
|
Solution C | 20 | ml | ||
|
Solution D | 1 | ml | ||
|
Solution E | 1 | ml | ||
1 Solutions A, B, and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solution E is prepared under a 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust to pH 7.0-7.2, if necessary. |
Compound | Amount | Unit | |
---|---|---|---|
|
NaCl | 25.0 | g |
|
KH2PO4 | 2.0 | g |
|
KNO3 | 2.0 | g |
|
NH4Cl | 1.0 | g |
|
MgSO4 x 7 H2O | 0.8 | g |
|
Trace element solution SL-4 | 2.0 | ml |
|
Distilled water | 940.0 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Na2-EDTA | 0.50 | g | ||
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
H3BO3 | 0.30 | g | ||
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
Compound | Amount | Unit | |
---|---|---|---|
|
Biotin | 20 | mg |
|
Folic acid | 20 | mg |
|
Pyridoxine hydrochloride | 100 | mg |
|
Thiamine HCl | 50 | mg |
|
Riboflavin | 50 | mg |
|
Nicotinic acid | 50 | mg |
|
Calcium D-(+)-pantothenate | 50 | mg |
|
Vitamin B12 | 1 | mg |
|
p-Aminobenzoic acid | 50 | mg |
|
(DL)-alpha-Lipoic acid | 50 | mg |
|
Distilled water | 1000 | ml |