1144
1144 .
SINGULISPHAERA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1144 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| N-Acetylglucosamine | 1.0 | g | 1 | 4.521 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.735 | |
| Hutners basal salts | 20.0 | ml | - | - | |
|
Na-ampicillin
(may be omitted for pure cultures) |
0.2 | g | 0.2 | 0.539 | |
| Peptone | 0.1 | g | 0.1 | - | |
| Yeast extract | 0.1 | g | 0.1 | - | |
|
Agar
(Difco) |
18.0 | g | 18 | 53.519 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 5.8. | |||||
Solution occurs in 3
other media (first defined in Medium 1144).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.70 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.34 | 22.719 | |
| (NH4)6Mo7O24 x 4 H2O | 9.25 | mg | 0.009 | - | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.00 | ml | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.8 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 18 | 53.519 |
| N-Acetylglucosamine | 1 | 4.521 |
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Na-ampicillin | 0.2 | 0.539 |
| Show more | ||
