Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 30 | 513.347 | |
KCl | 0.20 | g | 0.2 | 2.683 | |
K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
Yeast extract | 0.50 | g | 0.5 | - | |
Peptone | 5.00 | g | 5 | - | |
Trace element solution (mg per 200 ml) | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the medium omitting Na2CO3, NaHCO3, Na2S x 9 H2O, and Vitamin solution. | |||||
2 Boil the medium and cool under nitrogen, add Na2CO3 and NaHCO3. | |||||
NaHCO3 | 24.00 | g | 24 | 285.69 | |
Na2CO3 | 2.76 | g | 2.76 | 26.041 | |
3 Adjust pH to 9.5-10.0 with 10M KOH. | |||||
4 Dispense into tubes or bottle fitted with rubber stoppers. | |||||
5 Autoclave at 121 °C for 15 min. | |||||
6 Add Na2S x 9 H2O (neutralized) and Vitamin solution from sterile stock solutions | |||||
Na2S x 9 H2O
(from neutralized stock solution) |
0.40 | g | 0.4 | 1.665 | |
Wolin's vitamin solution | 2.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MnCl2 x 4 H2O | 720 | mg | 3.6 | 18.191 |
Fe(NH4)2(SO4)2 x 12 H2O | 400 | mg | 2 | - |
FeSO4 x 7 H2O | 200 | mg | 1 | 3.597 |
CoCl2 x 6 H2O | 200 | mg | 1 | 7.702 |
ZnSO4 x 7 H2O | 200 | mg | 1 | 3.478 |
Na2MoO4 x 2 H2O | 20 | mg | 0.1 | 0.413 |
NiCl2 | 100 | mg | 0.5 | 3.858 |
CuSO4 x 5 H2O | 20 | mg | 0.1 | 0.401 |
AlK(SO4)2 x 12 H2O | 20 | mg | 0.1 | 0.211 |
H3BO3 | 20 | mg | 0.1 | 1.617 |
HCl
(concentrated) |
5 | ml | - | - |
Distilled water | 200 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 03.02.23 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 9.5 - 10.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 30 | 513.347 |
NaHCO3 | 24 | 285.69 |
Peptone | 5 | - |
Na2CO3 | 2.76 | 26.041 |
NH4Cl | 1 | 18.695 |
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