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DSMZ

DSMZ 1149 . OCEANITHERMUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1149 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.78 4.478
KH2PO4 0.75 g 0.75 5.511
Trace element solution 10.00 ml - -
Vitamin solution 10.00 ml - -
MgCl2 x 6 H2O 0.36 g 0.36 1.771
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NH4Cl 0.53 g 0.53 9.908
Na2SO4 0.14 g 0.14 0.986
NaCl 20.00 g 20 342.231
Yeast extract 2.00 g 2 -
NaNO3 1.70 g 1.7 20.001
Distilled water 1000.00 ml - -
1 Prepare the medium without vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Vitamins (filter sterilized and stored under nitrogen) and 100 ml 0.6 M NaHCO3 solution (sterilized and stored under nitrogen) are added from stock solutions.
2 The pH should be pH 6.5.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.800 g 12.8 66.967
FeCl3 x 6 H2O 1.350 g 1.35 4.995
MnCl2 x 4 H2O 0.100 g 0.1 0.505
CoCl2 x 6 H2O 0.024 g 0.024 0.185
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.025 g 0.025 0.147
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NaCl 1.000 g 1 17.112
NiCl2 x 6 H2O 0.120 g 0.12 0.926
Na2SeO3 x 5 H2O 0.026 g 0.026 0.099
Distilled water 1000.000 ml - -

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
Yeast extract 2 -
NaNO3 1.7 20.001
K2HPO4 0.78 4.478
KH2PO4 0.75 5.511
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain