This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 27.70 | g | 27.7 | 473.99 | |
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
MgCl2 x 6 H2O | 5.50 | g | 5.5 | 27.053 | |
KH2PO4 | 1.00 | g | 1 | 7.348 | |
CaCl2 x 2 H2O | 0.75 | g | 0.75 | 5.102 | |
KCl | 0.66 | g | 0.66 | 8.853 | |
(NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
NaBr | 0.10 | g | 0.1 | 0.972 | |
H3BO3 | 30.00 | mg | 0.03 | 0.485 | |
SrCl2 x 6 H2O | 15.00 | mg | 0.015 | 0.056 | |
KJ
(1 mg/ml) |
50.00 | µl | - | - | |
Elemental sulfur | 10.00 | g | 10 | - | |
Double distilled water | 1000.00 | ml | - | - | |
Na2S
, used for reduction of the medium
(2.5 %) |
20.00 | ml | 0.5 | 6.324 | |
Yeast extract | 0.04 | % | 0.4 | - | |
1 Reduction of the medium by addition of 20 ml Na2S (2.5%); then adjust pH to 5.5 with sulfuric acid. | |||||
2 Gas phase: H2/CO2=80/20 (250 kPa). | |||||
3 After autoclaving add 0.04% yeast extract. | |||||
4 Incubation at 90°C for 1-2 days. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | 5.5 |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 27.7 | 473.99 |
Elemental sulfur | 10 | - |
MgSO4 x 7 H2O | 7 | 28.4 |
MgCl2 x 6 H2O | 5.5 | 27.053 |
KH2PO4 | 1 | 7.348 |
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