1156
1156 .
HALOPHILIC METHANOTROPHIC BACTERIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1156 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| H2O | 1000.00 | ml | - | - | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Na2HPO4 x 2 H2O | 0.70 | g | 0.7 | 4.931 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.205 | |
| NaCl | 65.00 | g | 65 | 1112.25 | |
| CaCl2 x 6 H2O | 0.01 | g | 0.01 | 0.046 | |
| FeSO4 x 7 H2O | 5.00 | mg | 0.005 | 0.018 | |
| Trace Element Solution | 1.00 | ml | - | - | |
| 1 pH-value 7.2 (Adjust to 7.6 with 10% NaOH before sterilization) | |||||
| 2 Culture bottles: 120-ml Serum bottles with 25 ml medium closed with butyl rubber serum caps and aluminium crimps | |||||
| 3 Gas supply: 2 vol.% CO2; 20 vol.% CH4; 78 vol.% air (Alternate cultivation method: instead of a methane-containing headspace, add 0.5% (w/v) methanol to the medium) | |||||
| 4 Culture conditions: 30°C; shaking culture on rotary shaker (200 rpm) | |||||
| 5 Notes: pH is critical, final pH must be between 6.5-7.5; some insoluble phosphates may precipitate during medium preparation (should not be more than OD 0.005). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.44 | mg | 0.44 | 1.53 |
| CuSO4 x 5 H2O | 0.20 | mg | 0.2 | 0.801 |
| MnSO4 x 2 H2O | 0.17 | mg | 0.17 | 0.909 |
| Na2MoO4 x 2 H2O | 0.06 | mg | 0.06 | 0.248 |
| H3BO3 | 0.10 | mg | 0.1 | 1.617 |
| CoCl2 x 6 H2O | 0.08 | mg | 0.08 | 0.616 |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.5 - 7.5 |
| Edit in Medium builder | |
Gas composition
- 78 % Air
- 20 % CH4
- 2 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 65 | 1112.25 |
| Na2HPO4 x 2 H2O | 0.7 | 4.931 |
| NH4Cl | 0.5 | 9.348 |
| KH2PO4 | 0.3 | 2.205 |
| MgSO4 x 7 H2O | 0.1 | 0.406 |
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