1162
1162 .
THIOPHAEOCOCCUS MANGROVI MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1162 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
| MgSO4 x 7 H2O | 2.00 | g | 2 | 8.114 | |
| NH4Cl | 0.64 | g | 0.64 | 11.965 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
| Yeast extract | 0.40 | g | 0.4 | - | |
| Sodium pyruvate | 3.00 | g | 3 | 27.263 | |
| Micronutrient solution SL 8 | 1.00 | ml | - | - | |
|
NaHCO3
(10% w/v) |
10.00 | ml | 1 | 11.904 | |
|
Vitamin B12
(2 mg/ml w/v) |
1.00 | ml | - | - | |
| Na2S x 9 H2O | 1.00 | mM | 0.24 | 1 | |
| Na2S2O3 x 5 H2O | 6.00 | mM | 1.489 | 6 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust to pH 7.5 | |||||
| 2 Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. | |||||
| 3 Notes: Filter-sterilized Vitamin B12, Na2S x 9 H2O, Na2S2O3 x 5 H2O are to be added only after autoclaving the medium. | |||||
Solution occurs in 2
other media (first defined in Medium 1162).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 5.2 | g | - | - |
| FeCl2 x 4 H2O | 1.5 | g | - | - |
| ZnCl2 | 70.0 | mg | - | - |
| MnCl2 x 4 H2O | 100.0 | mg | - | - |
| H3BO3 | 62.0 | mg | - | - |
| CoCl2 x 6 H2O | 190.0 | mg | - | - |
| CuCl2 x 2 H2O | 17.0 | mg | - | - |
| NiCl2 x 6 H2O | 24.0 | mg | - | - |
| Na2MoO4 x 2 H2O | 36.0 | mg | - | - |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 20 | 342.231 |
| Sodium pyruvate | 3 | 27.263 |
| MgSO4 x 7 H2O | 2 | 8.114 |
| Na2S2O3 x 5 H2O | 1.489 | 6 |
| NaHCO3 | 1 | 11.904 |
| Show more | ||
