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DSMZ

DSMZ 1165 . SPIROCHAETA AMERICANA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Alkalispirochaeta americana DSM 14872

This strain uses the standard medium without modifications.

Growth conditions: 37 °C

Main sol. 1165 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
Na2CO3 2.76 g 2.76 26.041
NaHCO3 24.00 g 24 285.69
KCl 0.20 g 0.2 2.683
K2HPO4 0.20 g 0.2 1.148
MgCl2 x 6 H2O 0.10 g 0.1 0.492
NH4Cl 1.00 g 1 18.695
Na2S x 9 H2O 0.40 g 0.4 1.665
Yeast extract 0.50 g 0.5 -
D-Glucose 5.00 g 5 27.753
Vitamin solution 2.00 ml - -
Trace element solution 1.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 1000.00 ml - -
1 Adjust to pH 9.4
2 Prepare the medium without the Na2S, yeast extract, glucose, Na2CO3, NaHCO3 and vitamins. Heat the medium in a boiling water bath and cool under a stream of nitrogen. Add the Na2CO3, and NaHCO3 to the cooled medium and check the pH. Dispense into appropriate vessels (Hungate tubes or serum bottles) and autoclave. To the sterile medium add Na2S, yeast extract, and glucose from autoclaved stock solutions. The vitamin solution is filter sterilised and added to the sterilised medium.

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace element solution #

200
Equipment needed: Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 720 mg 3.6 18.191
Fe(NH4)2(SO4)2 x 12 H2O 400 mg 2 -
FeSO4 x 7 H2O 200 mg 1 3.597
CoCl2 x 6 H2O 200 mg 1 7.702
ZnSO4 x 7 H2O 200 mg 1 3.478
Na2MoO4 x 2 H2O 20 mg 0.1 0.413
NiCl2 100 mg 0.5 3.858
CuSO4 x 5 H2O 20 mg 0.1 0.401
AlK(SO4)2 x 12 H2O 20 mg 0.1 0.211
H3BO3 20 mg 0.1 1.617
HCl 5 ml - -
Distilled water 200 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.4
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1
Alkalispirochaeta americana DSM 14872

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 24 285.69
D-Glucose 5 27.753
Na2CO3 2.76 26.041
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain