DSMZ 1166 . DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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* Modified for Desulfurispira natronophila DSM 22071

Replace formate and pyruvate with 1.60 g/l Na-acetate as substrate and supplement medium with 3.00 g/l sulfur. For sterilization steam sulfur for 3 hours on each of 3 successive days. Add sulfur aseptically to the autoclaved medium while retaining anoxic conditions.

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.00 g 5.982 102.362
K2HPO4 1.00 g 0.997 5.724
Na2SO4 2.80 g 2.792 19.654
NaHCO3 8.00 g 7.976 94.945
Na2CO3 22.00 g 21.934 206.95
NH4Cl 0.20 g 0.199 3.728
MgCl2 x 6 H2O 0.20 g 0.199 0.981
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 0.05 g 0.05 -
Na-formate* 3.40 g 3.39 49.845
Na-pyruvate* 0.55 g 0.548 4.983
Na2S2O3 x 5 H2O 5.00 g 4.985 20.086
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.24 g 0.239 0.996
Elemental sulfur* 3.00 g - -
Na acetate* 1.60 g - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, hydrogenphosphate and sodium sulfate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, formate, pyruvate, thiosulfate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of vitamins and thiosulfate should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 10.
Solution occurs in 27 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0
Solution occurs in 176 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 16.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.5 - 10.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 17
Strains with modifications: 12
Desulfurispira natronophila DSM 22071

  3 g Elemental sulfur
  1.6 g Na acetate
  Na-pyruvate
  Na-formate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.934 206.95
NaHCO3 7.976 94.945
NaCl 5.982 102.362
Na2S2O3 x 5 H2O 4.985 20.086
Elemental sulfur 2.991 -
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Cultivation metadata from 17 strains BacDive

pH 8 - 10 3 strains
pH 10 - 12 3 strains
phyla 4 16 strains
oxygen anaerobe 13 strains
oxygen obligate anaerobe 1 strain
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