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DSMZ

DSMZ 1170 . METHYLONATRUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methylonatrum kenyense DSM 15732

This strain uses the standard medium without modifications.

Growth conditions: 7-14 days aerobic 28 °C

Main sol. 1170 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 1.00 g 1 5.741
KNO3 1.00 g 1 9.891
Na2CO3 11.00 g 11 103.785
Methanol 2.00 ml 1.584 49.435
NaCl 18.00 g 18 308.008
NH4Cl 0.16 g 0.16 2.991
MgSO4 x 7 H2O 0.24 g 0.24 0.974
NaS2O3 x 5 H2O 24.00 mg 0.024 -
Trace element solution SL-6 1.00 ml - -
Vitamin B12 100.00 mg 0.1 0.074
Distilled water 1000.00 ml - -
1 pH 10.0
2 Prepare the medium without methanol, vitamin B12, MgSO4 x 7 H2O and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20 mM sodium acetate instead of methanol.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Methylonatrum kenyense DSM 15732

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18 308.008
Na2CO3 11 103.785
Methanol 1.584 49.435
KNO3 1 9.891
K2HPO4 1 5.741
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain