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DSMZ

DSMZ 1171 . METHYLOHALOMONAS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methylohalomonas lacus DSM 15733

This strain uses the standard medium without modifications.

Growth conditions: 7-14 days aerobic 28 °C pH 7.50

Main sol. 1171 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.000 g 2 11.483
NH4Cl 0.500 g 0.5 9.348
NaCl 120.000 g 120 2053.39
MgSO4 x 7 H2O 0.500 g 0.5 2.029
NaS2O3 x 5 H2O 0.024 g 0.024 -
Trace element solution SL-6 1.000 ml - -
NaHCO3 1.700 g 1.7 20.236
Vitamin B12 100.000 µg 1.0e-4 7.4e-5
Methanol 2.000 ml 1.584 49.435
Distilled water 1000.000 ml - -
1 pH 7.5
2 Prepare the medium without methanol, vitamin B12 MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20mM sodium acetate instead of methanol.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Methylohalomonas lacus DSM 15733

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 120 2053.39
K2HPO4 2 11.483
NaHCO3 1.7 20.236
Methanol 1.584 49.435
NH4Cl 0.5 9.348
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain