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1171. METHYLOHALOMONAS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.000 g 1.994 11.448
2 0.500 g 0.499 9.319
3 120.000 g 119.641 2047.16
4 0.500 g 0.499 2.023
5 0.024 g 0.024 -
6 Trace element solution SL-6 1.000 ml - -
7 1.700 g 1.695 20.176
8 100.000 µg 1.0e-4 6.3e-5
9 2.000 ml 1.575 49.163
10 1000.000 ml 995.221 55243.1
Instructions:
  1. pH 7.5
  2. Prepare the medium without methanol, vitamin B12 MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20mM sodium acetate instead of methanol.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Methylohalomonas lacus DSM 15733

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.216 55298.3
NaCl 119.641 2047.16
K2HPO4 1.994 11.448
NaHCO3 1.695 20.176
Methanol 1.575 49.163
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Cultivation metadata from 1 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
halophily slightly 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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