1171 .
METHYLOHALOMONAS MEDIUM
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Selected strain: Methylohalomonas lacus DSM 15733
This strain uses the standard medium without modifications.
Growth conditions: 7-14 days
aerobic
28 °C
pH 7.50
Equipment needed:
Autoclave
Filter
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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K2HPO4 | 2.000 | g | ||
|
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NH4Cl | 0.500 | g | ||
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NaCl | 120.000 | g | ||
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MgSO4 x 7 H2O | 0.500 | g | ||
|
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NaS2O3 x 5 H2O | 0.024 | g | ||
|
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Trace element solution SL-6 | 1.000 | ml | ||
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NaHCO3 | 1.700 | g | ||
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Vitamin B12 | 100.000 | µg | ||
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Methanol | 2.000 | ml | ||
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Distilled water | 1000.000 | ml | ||
| 1 pH 7.5 | |||||
| 2 Prepare the medium without methanol, vitamin B12 MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20mM sodium acetate instead of methanol. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
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MnCl2 x 4 H2O | 0.03 | g |
|
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H3BO3 | 0.30 | g |
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CoCl2 x 6 H2O | 0.20 | g |
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CuCl2 x 2 H2O | 0.01 | g |
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NiCl2 x 6 H2O | 0.02 | g |
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Na2MoO4 x 2 H2O | 0.03 | g |
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Distilled water | 1000.00 | ml |