This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 120.00 | g | 120 | 2053.39 | |
K2HPO4 | 3.00 | g | 3 | 17.224 | |
NH4Cl | 0.50 | g | 0.5 | 9.348 | |
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
HEPES | 2.38 | g | 2.38 | 9.987 | |
Distilled water | 1000.00 | ml | - | - | |
1 After sterilization add from sterile stock solutions add: | |||||
MgSO4 x 7 H2O
(1M) |
2.00 | ml/l | 0.493 | 2 | |
Sodium acetate
(2M) |
10.00 | ml/l | 1.641 | 20 | |
Yeast extract
(10%) |
2.00 | ml/l | 0.2 | - | |
2 Adjust pH to 7.5. Dispense the medium into sealed bottles and autoclave. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 5.00 | g | 5 | 17.109 | |
FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.03 | g | 0.03 | 0.485 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 pH 3.0-4.0 |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 120 | 2053.39 |
K2HPO4 | 3 | 17.224 |
HEPES | 2.38 | 9.987 |
Sodium acetate | 1.641 | 20 |
NH4Cl | 0.5 | 9.348 |
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