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1187. ANOYXNATRONUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25.0 g 24.95 235.405
2 25.0 g 24.95 297.002
3 0.2 g 0.2 2.677
4 0.1 g 0.1 0.491
5 0.5 g 0.499 9.329
6 0.2 g 0.2 1.146
7 0.2 g 0.2 -
8 Trace element solution SL-10 1.0 ml - -
9 Vitamin solution 1.0 ml - -
10 0.7 g 0.699 2.909
11 5.0 g 4.99 27.698
12 1000.0 ml 996.215 55298.2
Instructions:
  1. pH 9.0
  2. Prepare the medium anaerobically without the vitamins, Na2S, yeast extract NaHCO3, and Na2CO3. Boil the medium, cool under N2, add the NaHCO3, and Na2CO3, dispense under N2 and autoclave the medium. Add the vitamin solution, yeast extract, Na2S and glucose from sterile stock solution prepared under N2 to the cooled, autoclaved medium.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.0
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
NaHCO3 24.95 297.002
Na2CO3 24.95 235.405
Glucose 4.99 27.698
Na2S x 9 H2O 0.699 2.909
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