Name: SPIROCHAETA CELLOBIOSIPHILA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1188 #

Equipment needed: Hungate tubes Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Sea Salt (SIGMA)	35.0	g	34.965	-
Wolfe's mineral elixir	1.0	ml	-	-
Yeast extract	1.0	g	0.999	-
Trypticase peptone (BD BBL)	0.5	g	0.5	-
Cellobiose	2.0	g	1.998	5.837
Distilled water	1000.0	ml	-	-
1 Dissolve compounds except cellobiose, adjust pH to 7.5 and dispense medium under air atmosphere into Hungate-type tubes or serum vials to 30% volume and autoclave. Add cellobiose after autoclaving from a stock solution sterilized by filtration.

Wolfe's mineral elixir #

1000

Solution occurs in 21 other media (first defined in Medium 792).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgSO₄ x 7 H₂O	30.00	g	30	121.716
MnSO₄ x H₂O	5.00	g	5	29.583
NaCl	10.00	g	10	171.116
FeSO₄ x 7 H₂O	1.00	g	1	3.597
CoCl₂ x 6 H₂O	1.80	g	1.8	13.863
CaCl₂ x 2 H₂O	1.00	g	1	6.802
ZnSO₄ x 7 H₂O	1.80	g	1.8	6.26
CuSO₄ x 5 H₂O	0.10	g	0.1	0.401
AlK(SO₄)₂ x 12 H₂O	0.18	g	0.18	0.379
H₃BO₃	0.10	g	0.1	1.617
Na₂MoO₄ x 2 H₂O	0.10	g	0.1	0.413
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	2.80	g	2.8	7.089
Na₂WO₄ x 2 H₂O	0.10	g	0.1	0.303
Na₂SeO₄	0.10	g	0.1	0.529
Distilled water	1000.00	ml	-	-
1 First adjust pH to 1.0 with diluted H₂SO₄, then add and dissolve the salts.

Last modified:	13.04.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.5
Equipment needed:	Autoclave, Filter, Hungate tubes

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1188	SPIROCHAETA CELLOBIOSIPHILA MEDIUM
b	MARISPIROCHAETA MEDIUM