DSMZ 118 . DAP-NUTRIENT AGAR

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Equipment needed: Autoclave

Final pH: 7.0

Select a strain (optional):

ml
Compound Amount Unit
Peptone 5 g
Meat extract 3 g
Diaminopimelic acid 100 mg
Agar , if necessary 15 g
Distilled water 1000 ml
1 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.