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DSMZ

DSMZ 1190 . FERROUS SULFATE/YEAST EXTRACT MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1190 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.2 g 0.2 -
50x Heterotrophic basal salts solution 20.0 ml - -
Ferrous sulfate solution (27.8%) 20.0 ml - -
Distilled water 960.0 ml - -
1 Dissolve yeast extract in distilled water and add basal salts solution. Adjust to desired pH with H2SO4. Dispense in suitable cultivation vessels and autoclave at 121°C for 20 min. When cool, add filter sterilized ferrous sulfate solution.

50x Heterotrophic basal salts solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 x 10 H2O 7.5 g 7.5 23.278
(NH4)2SO4 22.5 g 22.5 170.274
KCl 2.5 g 2.5 33.535
MgSO4 x 7 H2O 25.0 g 25 101.43
KH2PO4 2.5 g 2.5 18.371
Ca(NO3)2 x 4 H2O 0.7 g 0.7 2.964
Distilled water 1000.0 ml - -

Ferrous sulfate solution (27.8%) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 278 g 278 999.946
Distilled water 1000 ml - -
1 Adjust pH to 1.8 with H2SO4; sterilize by filtration.

Metadata

Last modified: 13.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 10
Strains with modifications: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 5.56 19.999
MgSO4 x 7 H2O 0.5 2.029
(NH4)2SO4 0.45 3.405
Yeast extract 0.2 -
Na2SO4 x 10 H2O 0.15 0.466
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Cultivation metadata from 10 strains BacDive

pH 0 - 2 2 strains
phyla 3 9 strains
temperature mesophilic 5 strains
temperature thermophilic 4 strains