DSMZ 1193 . CULTIVATION MEDIUM FOR CHLAMYDIAE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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* Modified for Chlamydia trachomatis DSM 19440

Plus 5% CO2

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Equipment needed: Filter Centrifuge
1 Cultivation of L929 (ACC 2) or HeLa (ACC 57) cells
Compound Amount Unit Conc. [g/L] Conc. [mM]
IMDM-Medium 45.0 ml - -
Fetal bovine serum 5.0 ml - -
Aminoacids
(100 x)
0.5 ml - -
2 Filter-sterilize (0.2 µm) and keep no longer than 4 weeks. Store at room temperature to facilitate detection of contamination.
3 Prepare a 25 cm2 flask and seed cells according to standard protocols (see DSMZ catalogue for ACC 2 and ACC 57). Incubate at 37°C plus 5% CO2. When a confluent layer has formed, infection can be carried out.
4 Exchange medium with 6 ml of Infection Medium (as above with the addition of 1 µg/ml cycloheximide (final concentration)) and add 500 - 1000 µl of EB stock solution (thawed quickly to 37°C).
5 Centrifuge for 1 h onto the cell layer at 1600 rpm at 20°C.
6 Incubate at 37°C + 5% CO2. Control cells daily and look for inclusions. Not all Chlamydiae form well-visible inclusions, ultimately, immunofluorescence or in situ-hybridization techniques are necessary to visualize inclusions.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Centrifuge, Filter
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Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 18
Strains with modifications: 17
Chlamydia trachomatis DSM 19440

  5% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Aminoacids - -
Fetal bovine serum - -
IMDM-Medium - -

Cultivation metadata from 18 strains BacDive

phyla 2 18 strains
temperature mesophilic 18 strains