Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
MgSO4 x 7 H2O | 0.40 | g | 0.4 | 1.623 | |
NaCl | 0.40 | g | 0.4 | 6.845 | |
NH4Cl | 0.40 | g | 0.4 | 7.478 | |
CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
FeSO4 x 7 H2O solution (0.1% w/v) | 2.00 | ml | - | - | |
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
Na-acetate | 1.00 | g | 1 | 12.19 | |
Na-formate | 2.00 | g | 2 | 29.409 | |
Sludge fluid | 50.00 | ml | - | - | |
Fatty acid mixture | 20.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
NaHCO3 | 0.50 | g | 0.5 | 5.952 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 930.00 | ml | - | - | |
1 Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 4 | g | 4 | - | |
Sludge | 1000 | ml | - | - | |
1 Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at 8-12°C in the dark. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Isobutyric acid | 23 | ml | - | - | |
DL-2-Methylbutyric acid | 27 | ml | - | - | |
Valeric acid | 27 | ml | - | - | |
Isovaleric acid | 27 | ml | - | - | |
Distilled water | 896 | ml | - | - | |
1 Adjust pH to 7.5 with concentrated NaOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
FeSO4 x 7 H2O | 1 | g | 1 | 3.597 | |
H2SO4
(0.1 N) |
1000 | ml | - | - | |
1 The ferrous sulfate solution is not stable and should be freshly prepared. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.8 - 7.0 |
Equipment needed: | Autoclave, Centrifuge, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na-formate | 2 | 29.409 |
Na-acetate | 1 | 12.19 |
NaHCO3 | 0.5 | 5.952 |
KH2PO4 | 0.5 | 3.674 |
L-Cysteine HCl x H2O | 0.5 | 2.847 |
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