DSMZ 11a . MODIFIED MEDIUM 11

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 11 990 ml - -
Vitamin solution 10 ml - -
1 Prepare medium 11 with additional vitamins. Adjust pH to 6.2 - 6.5
Solution occurs in 1 other media (first defined in Medium 11).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone
(tryptic digest)
10.00 g 10 -
Meat extract 10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 20.00 g 20 111.012
Tween 80 1.00 g 1 0.764
K2HPO4 2.00 g 2 11.483
Na-acetate 5.00 g 5 60.951
(NH4)3 citrate 2.00 g 2 8.223
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x H2O 0.05 g 0.05 0.296
Distilled water 1000.00 ml - -
1 Adjust pH to 6.2 - 6.5.
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Metadata

Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 19.8 109.902
Meat extract 9.9 -
Casein peptone 9.9 -
Na-acetate 4.95 60.341
Yeast extract 4.95 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain