11a
11a .
MODIFIED MEDIUM 11
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Ligilactobacillus acidipiscis DSM 15836
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 11a #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 11 | 990 | ml | - | - | |
| Vitamin solution | 10 | ml | - | - | |
| 1 Prepare medium 11 with additional vitamins. Adjust pH to 6.2 - 6.5 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
| Meat extract | 10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Glucose | 20.00 | g | 20 | 111.012 | |
| Tween 80 | 1.00 | g | 1 | 0.764 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| Na-acetate | 5.00 | g | 5 | 60.951 | |
| (NH4)3 citrate | 2.00 | g | 2 | 8.223 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.2 - 6.5. | |||||
Solution occurs in 3
other media (first defined in Medium 11a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.00 | mg | 0.002 | 0.008 |
| Folic acid | 2.00 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.00 | mg | 0.01 | 0.049 |
| Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | 0.013 |
| Riboflavin | 5.00 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.00 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 |
| p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 10.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Ligilactobacillus acidipiscis DSM 15836
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 19.8 | 109.902 |
| Meat extract | 9.9 | - |
| Casein peptone | 9.9 | - |
| Na-acetate | 4.95 | 60.341 |
| Yeast extract | 4.95 | - |
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