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11b. MRS MEDIUM pre-reduced

Culture medium recipe

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* Modified for Selenomonas lacticifex DSM 20757
beta

Anaerobic

Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.00 g 10 -
2 10.00 g 10 -
3 5.00 g 5 -
4 20.00 g 20 111.015
5 1.00 g 1 0.764
6 1.00 mg 1.0e-3 0.004
7 2.00 g 2 11.483
8 5.00 g 5 60.951
9 2.00 g 2 8.223
10 0.20 g 0.2 0.811
11 0.05 g 0.05 0.296
12 1000.00 ml 998.207 55408.8
13 0.50 g 0.5 2.847
Instructions:
  1. Cysteine is added after the medium has been boiled and cooled under CO2. Adjust pH to 6.2 - 6.5, distribute under N2 and autoclave.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.3
Equipment needed: Autoclave

Associated strains

All strains for this medium: 9
Strains with modifications: 7
Selenomonas lacticifex DSM 20757

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Glucose 20 111.015
Casein peptone, tryptic digest 10 -
Meat extract 10 -
Sodium acetate 5 60.951
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Cultivation metadata from 9 strains BacDive

pH 6 - 8 3 strains
phyla 1 9 strains
oxygen anaerobe 8 strains
oxygen microaerophile 1 strain
temperature mesophilic 9 strains

Similar media

11a. MODIFIED MEDIUM 11 96 %
11. MRS MEDIUM 96 %
231. PEDIOCOCCUS DAMNOSUS MEDIUM 94 %
232. MRS MEDIUM WITH CYSTEINE 89 %
232b. WEISSELA KOREENSIS MEDIUM 88 %

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