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12. SOIL EXTRACT MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Centrifuge
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(with high content of organic matter)
400 g 400 -
2 1000 ml - -
3 15 g/l 15 -
Instructions:
  1. Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Centrifuge

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Air-dried garden soil 400 -
Agar 15 -
Tap water - -

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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