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1201. ALICYCLOBACILLUS FERROOXYDANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 930 ml - -
2 Solution B 70 ml - -
Instructions:
  1. Autoclave solution A, cool down to 50°C and combine solution A with 70 ml of solution B.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.538 2.181
2 0.40 g 0.43 3.255
3 0.20 g 0.215 1.235
4 0.15 g 0.161 0.533
5 0.10 g 0.108 1.442
6 0.01 g 0.011 0.064
7 0.20 g 0.215 -
8 930.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 1.8 - 2.5 with H2SO4.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20 g 200 719.386
2
(0.2 N)
100 ml - -
Instructions:
  1. Dissolve in 0.2 N H2SO4 and fill up to a volume of 100 ml. Filter sterilize.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 2.1
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
FeSO4 x 7 H2O 15.054 54.147
MgSO4 x 7 H2O 0.538 2.181
(NH4)2SO4 0.43 3.255
K2HPO4 0.215 1.235
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 0 - 2 1 strain
pH 2 - 4 1 strain
pH 4 - 6 1 strain
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