1203
1203 .
FASTIDIOUS ANAEROBE AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Clostridioides difficile DSM 27543
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 1203 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Fastidious Anaerobe Agar
(acumedia) |
45.7 | g | 45.7 | - | |
| Deionized water | 1000.0 | ml | - | - | |
| Horse blood , adjust if required | 10.0 | % | 100 | - | |
| 1 Allow to soak for 10 minutes, swirl to mix then sterilize by autoclaving at 121°C for 15 minutes. Cool to 47°C then aseptically add 5-10% of sterile defibrinated horse blood, mix well and pour plates. pH: 7.2 ± 0.2 | |||||
Metadata
| Last modified: | 31.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Taxonomic range: | Phages |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.4 |
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Associated strains
| All strains for this medium: | 92 |
| Strains with modifications: | 4 |
Clostridioides difficile DSM 27543
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Horse blood | 100 | - |
| Fastidious Anaerobe Agar | 45.7 | - |
| Deionized water | - | - |
Cultivation metadata from 92 strains
| pH | 6 - 8 | 5 strains |
| pH | 4 - 6 | 1 strain |
| phyla | 10 | 86 strains |
| oxygen | anaerobe | 71 strains |
| oxygen | microaerophile | 5 strains |
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