Loading ...

1204. SPHAEROCHAETA MEDIUM

Culture medium recipe

Vol.
×
Go back to medium without modifications
* Modified for Spirochaeta coccoides DSM 17374
beta

Anaerobic

Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 1 17.111
2 0.50 g 0.5 6.707
3 0.30 g 0.3 5.608
4 0.20 g 0.2 1.47
5 0.15 g 0.15 1.056
6 0.40 g 0.4 1.968
7 0.10 g 0.1 0.68
8 4.00 g 4 -
9 2.00 g 2 -
10
(0.1% w/v)
0.50 ml 5.0e-4 0.002
11 1.00 g 1 9.435
12 2.00 g 2 5.551
13 0.80 g 0.8 5.186
14 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve ingredients except carbonate, maltose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 minutes. Add maltose and DTT from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Check pH of complete medium and adjust to 7.4, if necessary.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1
Spirochaeta coccoides DSM 17374

Gas composition

  • 20 % CO2
  • 80 % N2

Light

Intensity lux

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Yeast extract 4 -
Maltose x H2O 2 5.551
Caseine peptone 2 -
NaCl 1 17.111
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

Similar media

1687. THERMOANAEROBACULUM MEDIUM 69 %
1689. MEDIUM FOR SM250 60 %
1701. ANAEROBIC AZOSPIRILLUM MEDIUM 60 %
815a. DESULFOCELLA HALOPHILA MEDIUM 60 %
1084. METHERMICOCCUS (METHANOGEN) MEDIUM 59 %

Latest comments

No comments for this medium.