Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase soy broth
(BD BACTO) |
13.75 | g | 13.75 | - | |
Yeast extract | 1.00 | g | 1 | - | |
NaCl | 2.50 | g | 2.5 | 42.779 | |
D-Glucose | 3.60 | g | 3.6 | 19.982 | |
DL-Dithiothreitol
(DTT) |
0.15 | g | 0.15 | 0.972 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except glucose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.2 with HCl, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Add glucose and DTT from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. |
Last modified: | 25.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 4 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Trypticase soy broth | 13.75 | - |
D-Glucose | 3.6 | 19.982 |
NaCl | 2.5 | 42.779 |
Yeast extract | 1 | - |
DL-Dithiothreitol | 0.15 | 0.972 |
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