DSMZ 1205 . 1/2 TRYPTIC SOY BROTH (ANAEROBIC)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BD BACTO)
13.75 g 13.75 -
Yeast extract 1.00 g 1 -
NaCl 2.50 g 2.5 42.779
D-Glucose 3.60 g 3.6 19.982
DL-Dithiothreitol
(DTT)
0.15 g 0.15 0.972
Distilled water 1000.00 ml - -
1 Dissolve ingredients except glucose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.2 with HCl, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Add glucose and DTT from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration.

Metadata

Last modified: 25.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 13.75 -
D-Glucose 3.6 19.982
NaCl 2.5 42.779
Yeast extract 1 -
DL-Dithiothreitol 0.15 0.972
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Cultivation metadata from 4 strains BacDive

pH 6 - 8 2 strains
phyla 1 3 strains
oxygen anaerobe 2 strains
temperature mesophilic 3 strains