1205 .
1/2 TRYPTIC SOY BROTH (ANAEROBIC)
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Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth
(BD BACTO) |
13.75 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
NaCl | 2.50 | g | ||
|
|
D-Glucose | 3.60 | g | ||
|
|
DL-Dithiothreitol
(DTT) |
0.15 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except glucose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.2 with HCl, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Add glucose and DTT from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. | |||||