DSMZ 1208 . BACILLUS ALKALIDIAZOTROPHICUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 8.00 g 8 95.23
Na2CO3 22.00 g 22 207.571
NaCl 6.00 g 6 102.669
K2HPO4 1.00 g 1 5.741
NH4Cl 0.20 g 0.2 3.739
MgCl2 x 6 H2O 0.20 g 0.2 0.984
Yeast extract 0.05 g 0.05 -
Na2SO4 2.80 g 2.8 19.713
Trace element solution SL-11 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
Glucose 2.00 g 2 11.101
Distilled water 1000.00 ml - -
1 Dissolve ingredients except ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, boil medium for 1 min, then cool to room temperature under N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, from sterile, anoxic stock solution prepared under N2. The vitamin solution should be sterilized by filtration. Adjust pH of final medium to 10.
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 27.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
NaHCO3 8 95.23
NaCl 6 102.669
Na2SO4 2.8 19.713
Glucose 2 11.101
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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