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1208. BACILLUS ALKALIDIAZOTROPHICUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 8.00 g 7.905 94.101
2 22.00 g 21.739 205.109
3 6.00 g 5.929 101.448
4 1.00 g 0.988 5.673
5 0.20 g 0.198 3.695
6 0.20 g 0.198 0.972
7 0.05 g 0.049 -
8 2.80 g 2.767 19.479
9 Trace element solution SL-11 1.00 ml - -
10 Selenite-tungstate solution 1.00 ml - -
11 Vitamin solution 10.00 ml - -
12 2.00 g 1.976 10.97
13 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients except ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, boil medium for 1 min, then cool to room temperature under N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, from sterile, anoxic stock solution prepared under N2. The vitamin solution should be sterilized by filtration. Adjust pH of final medium to 10.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2CO3 21.739 205.109
NaHCO3 7.905 94.101
NaCl 5.929 101.448
Na2SO4 2.767 19.479
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Cultivation metadata from 1 strains BacDive

halophily moderate 1 strain
halophily not 1 strain
pH 10 - 14 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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