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120a. METHANOSARCINA BARKERI MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.35 g 0.342 1.962
2 0.23 g 0.225 1.651
3 0.50 g 0.488 9.128
4 0.50 g 0.488 1.981
5 0.25 g 0.244 1.661
6 2.25 g 2.197 37.597
7
(0.1% w/v in 0.1 N H2SO4)
2.00 ml 0.002 0.007
8 Trace element solution SL-10 1.00 ml - -
9
(OXOID)
2.00 g 1.953 -
10
(BD BBL)
2.00 g 1.953 -
11
(0.1% w/v)
0.50 ml 4.9e-4 0.002
12 , might need strain adjustment 0.85 g - -
13 Vitamin solution 10.00 ml - -
14 , might need strain adjustment 10.00 ml - -
15 0.30 g 0.293 1.668
16 0.30 g 0.293 1.22
17 1000.00 ml 974.812 54110.2
Instructions:
  1. Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Methanol (50% v/v stock solution) and the reducing agents are each autoclaved separately under 100% N2 gas atmosphere as concentrated solutions in tightly closed tubes. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Appropriate volumes of the solutions are injected into the sterile medium with hypodermic syringes. Adjust pH of the complete medium to 6.5 - 6.8, if necessary.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 14
Strains with modifications: 14

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 985.525 54704.9
NaCl 2.197 37.597
Yeast extract 1.953 -
Casitone 1.953 -
NH4Cl 0.488 9.128
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Cultivation metadata from 14 strains BacDive

phyla 2 14 strains
oxygen anaerobe 14 strains
temperature mesophilic 13 strains
temperature psychrophilic 1 strain

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