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DSMZ

DSMZ 121 . MEDIUM FOR SULFOSPIRILLUM DSM 806

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 121 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-aspartate 10.00 g 10 -
Yeast extract 0.20 g 0.2 -
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 28.00 mg 0.028 0.19
K2HPO4 0.75 g 0.75 4.306
NaH2PO4 0.25 g 0.25 2.084
L-Cysteine HCl x H2O 0.25 g 0.25 -
Resazurin 1.00 mg 1.0e-3 0.004
Trace element solution SL-10 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-aspartate 10 -
MgSO4 x 7 H2O 1 4.057
K2HPO4 0.75 4.306
NaH2PO4 0.25 2.084
L-Cysteine HCl x H2O 0.25 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain