MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 121 . MEDIUM FOR SULFOSPIRILLUM DSM 806

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 121 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-aspartate 10.00 g 10 -
Yeast extract 0.20 g 0.2 -
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 28.00 mg 0.028 0.19
K2HPO4 0.75 g 0.75 4.306
NaH2PO4 0.25 g 0.25 2.084
L-Cysteine HCl x H2O 0.25 g 0.25 -
Resazurin 1.00 mg 1.0e-3 0.004
Trace element solution SL-10 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-aspartate 10 -
MgSO4 x 7 H2O 1 4.057
K2HPO4 0.75 4.306
NaH2PO4 0.25 2.084
L-Cysteine HCl x H2O 0.25 -
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain