DSMZ 1210b . PYRODICTIUM DELANEYI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Centrifuge Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 18.00 g 17.946 307.087
MgCl2 x 6 H2O 4.00 g 3.988 19.616
KCl 0.33 g 0.329 4.413
CaCl2 x 2 H2O 0.33 g 0.329 2.238
(NH4)2SO4 0.50 g 0.499 3.773
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Na2CO3 1.00 g 0.997 9.407
KH2PO4 0.33 g 0.329 2.418
FeCl2 x 4 H2O 0.52 g 0.518 2.608
Yeast extract 0.20 g 0.199 -
Wolin's vitamin solution 1.00 ml - -
L-Cysteine HCl x H2O 0.10 g 0.1 0.568
Amorphous Fe(OH)3 100.00 ml - -
Distilled water 900.00 ml - -
1 Dissolve ingredients except carbonate, hydrogenphosphate, ferrous chloride, yeast extract, vitamins, cysteine and ferric iron hydroxide sludge. Suspend pellet of ferric iron hydroxide in medium and sparge with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Thereafter, dispense suspension under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 30 min. Add hydrogenphosphate, ferrous chloride (dissolved in 0.1 N HCl), yeast extract, cysteine and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and ferrous chloride should be sterilized by filtration. The pH of the complete medium should be at 5.5.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 2 other media (first defined in Medium 1210).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O
(60.00 g/l)
320 ml - -
NaOH , adjust if required
(10% w/v)
80 ml 20 500.038
1 Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10% (w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere.

Metadata

Last modified: 25.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 17.946 307.087
MgCl2 x 6 H2O 3.988 19.616
NaOH 1.994 49.854
Na2CO3 0.997 9.407
FeCl2 x 4 H2O 0.518 2.608
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition autotroph 1 strain
oxygen obligate anaerobe 1 strain
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