Loading ...

1210b. PYRODICTIUM DELANEYI MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 18.00 g 17.946 307.074
2 4.00 g 3.988 19.616
3 0.33 g 0.329 4.413
4 0.33 g 0.329 2.238
5 0.50 g 0.499 3.773
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 1.00 g 0.997 9.407
9 0.33 g 0.329 2.418
10 0.52 g 0.518 2.608
11 0.20 g 0.199 -
12 Vitamin solution 1.00 ml - -
13 0.10 g 0.1 0.568
14 Amorphous Fe(OH)3 100.00 ml - -
15 900.00 ml 895.699 49718.8
16 , required for dissolving ferrous chloride 0.10 N - -
Instructions:
  1. Dissolve ingredients except carbonate, hydrogenphosphate, ferrous chloride, yeast extract, vitamins, cysteine and ferric iron hydroxide sludge. Suspend pellet of ferric iron hydroxide in medium and sparge with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Thereafter, dispense suspension under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 30 min. Add hydrogenphosphate, ferrous chloride (dissolved in 0.1 N HCl), yeast extract, cysteine and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and ferrous chloride should be sterilized by filtration. The pH of the complete medium should be at 5.5.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 2 other media (first defined in medium 1210 )
Equipment needed: Centrifuge Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(60.00 g/l)
320 - -
2
(10% w/v)
80 ml 100 2500.18
3 x - -
Instructions:
  1. Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10%(w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 898.675 49884
NaCl 17.946 307.074
NaOH 9.971 249.283
MgCl2 x 6 H2O 3.988 19.616
Na2CO3 0.997 9.407
Show more

Cultivation metadata from 1 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
Show more

Similar media

1210. GEOGLOBUS MEDIUM 97 %
981. GEOTHERMOBACTER MEDIUM 92 %
846a. AMINOBACTERIUM MEDIUM 91 %
509. SPIROCHAETA THERMOPHILA MEDIUM 90 %
1210c. THERMOSULFURIPHILUS MEDIUM 90 %

Latest comments

No comments for this medium.