Name: MARINOSPIRILLUM CELERE
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1212 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	0.5	g	0.5	9.348
KH₂PO₄	0.5	g	0.5	3.674
NaCl	25.0	g	25	427.789
Na₂SO₄	0.5	g	0.5	3.52
MgSO₄ x 7 H₂O	0.2	g	0.2	0.811
KCl	0.5	g	0.5	6.707
Yeast extract	1.0	g	1	-
Peptone	1.0	g	1	-
Sodium DL-lactate	1.0	g	1	8.924
Vitamin B₁₂	15.0	µg	1.5e-5	1.1e-5
Na₂CO₃	5.0	g	5	47.175
NaHCO₃	5.0	g	5	59.519
Trace element solution SL-10 B	1.0	ml	-	-
Distilled water	1000.0	ml	-	-
Agar , for solid medium	15.0	g	15	44.599
1 Autoclave the Na₂CO₃, NaHCO₃ and the lactate separately from the medium and combine after cooling. Vitamin B₁₂ is added from a sterile stock solution. The final pH of the medium should be 9.5. Add 15.0 g/l agar for solid media.

Trace element solution SL-10 B #

1008

Solution occurs in 1 other media (first defined in Medium 1200).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Distilled water	1000.0	ml	-	-
HCl (25%)	7.7	ml	1.925	52.798
FeSO₄ x 7 H₂O	1.5	g	1.488	5.353
ZnCl₂	70.0	mg	0.069	0.509
MnCl₂ x 4 H₂O	100.0	mg	0.099	0.501
H₃BO₃	300.0	mg	0.298	4.813
CoCl₂ x 6 H₂O	190.0	mg	0.188	1.452
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.184
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.148

All strains for this medium:

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