Name: ROSEINATRONOBACTER MONICUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1218 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	0.4	g	0.4	7.478
KH₂PO₄	0.5	g	0.5	3.674
MgCl₂ x 6 H₂O	0.2	g	0.2	0.984
Na₂SO₄	0.5	g	0.5	3.52
NaNO₃	0.4	g	0.4	4.706
NaCl	40.0	g	40	684.463
KCl	0.5	g	0.5	6.707
NaHCO₃	10.0	g	10	119.038
Na₂CO₃	5.0	g	5	47.175
Na₂S₂O₃ x 5 H₂O	1.0	g	1	4.029
Vitamin B₁₂	10.0	µg	1.0e-5	7.4e-6
Sodium pyruvate	1.0	g	1	9.088
Sodium acetate	1.0	g	1	12.191
Peptone	1.0	g	1	-
Yeast extract	1.0	g	1	-
Trace element solution SL10	1.0	ml	-	-
Distilled water	1000.0	ml	-	-
1 Autoclave the NaHCO₃ and Na₂CO₃ separately and add to the medium. Add the vitamin B₁₂ solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final is pH 9.0 - 9.5

Trace element solution SL10 #

1005

Solution occurs in 1 other media (first defined in Medium 1217).

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (concentrated)	5.000	ml	-	-
FeCl₂ x 4 H₂O	1.500	g	1.493	7.507
ZnCl₂	0.070	g	0.07	0.511
MnCl₂ x 4 H₂O	0.200	g	0.199	1.006
H₃BO₃	6.000	mg	0.006	0.097
CoCl₂ x 6 H₂O	0.190	g	0.189	1.456
CuCl₂ x 2 H₂O	2.000	mg	0.002	0.012
NiCl₂ x 6 H₂O	0.024	g	0.024	0.184
Na₂MoO₄ x 2 H₂O	0.036	g	0.036	0.148
Distilled water	1000.000	ml	-	-

All strains for this medium:

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phyla	1	1 strain
temperature	mesophilic	1 strain