1219
1219 .
METHYLOVIRGULA LIGNI MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1219 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| Trace elements | 1.00 | ml | - | - | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Methanol | 1.00 | ml | 0.792 | 24.718 | |
| Distilled water | 1000.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 Adjust to pH 5.0 - 5.8. Prepare the medium in flasks or tubes with screw caps. The methanol may either be filter sterilised or autoclaved in half full serum bottles sealed with a rubber stopper and a crimp closure. Add to the cooled autoclaved medium. Agar may be added at 15 g/l for solid media. | |||||
Solution occurs in 2
other media (first defined in Medium 922).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 |
| CuCl2 x 5 H2O | 0.10 | g | 0.1 | 0.445 |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| Na2MoO4 | 0.03 | g | 0.03 | 0.146 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 5.8 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Methanol | 0.792 | 24.718 |
| (NH4)2SO4 | 0.1 | 0.757 |
| KH2PO4 | 0.1 | 0.735 |
| Yeast extract | 0.1 | - |
| Show more | ||
