DSMZ 122 . ACETIVIBRIO MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
MgCl2 x 6 H2O 2.60 g 2.6 12.789
KH2PO4 1.43 g 1.43 10.508
K2HPO4 5.50 g 5.5 31.577
CaCl2 x 2 H2O 0.13 g 0.13 0.884
Na2-ß-glycerophosphate x 5 H2O
(MERCK 35675)
6.00 g 6 19.601
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4)
1.10 ml 0.001 0.004
L-Glutathione
(reduced)
0.25 g 0.25 0.813
Yeast extract 4.50 g 4.5 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Cellobiose 5.00 g 5 14.607
Cellulose
(Avicel or MN 301)
optional
10.00 g 10 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL).
2 Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth.

Metadata

Last modified: 07.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellulose
optional
10 -
Na2-ß-glycerophosphate x 5 H2O 6 19.601
K2HPO4 5.5 31.577
Cellobiose 5 14.607
Yeast extract 4.5 -
Show more

Cultivation metadata from 9 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
phyla 1 9 strains
oxygen anaerobe 9 strains
temperature thermophilic 9 strains