1222
1222 .
RHODOVULUM MARINUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1222 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| NaCl | 20.00 | g | 20 | 342.231 | |
| NH4Cl | 0.60 | g | 0.6 | 11.217 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| Sorbitol OR Na pyruvate | 3.00 | g | 3 | 16.468 | |
| Trace element solution SL-8 | 1.00 | ml | - | - | |
|
Vitamin B12
(20mg/l) |
1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium under a nitrogen atmosphere, dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Add the vitamin B12 from a filter-sterilised stock solution. Either sorbitol or sodium pyruvate may serve as the carbon source. The final pH of the medium should be 6.8. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Distilled water | 1000.0 | ml | - | - | |
| Na2-EDTA | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| MnCl2 x 2 H2O | 100.0 | mg | 0.1 | - | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| H3BO3 | 62.0 | mg | 0.062 | 1.003 | |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 | |
| 1 Adjust pH to 6.8. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 6.8 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 20 | 342.231 |
| Na pyruvate | 3 | 27.262 |
| Sorbitol | 3 | 16.468 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| NH4Cl | 0.6 | 11.217 |
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