Loading ...

1224. ECTOTHIORHODOSPIRA VARIABILIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50.0 g 49.95 854.687
2 5.0 g 4.995 47.128
3 15.0 g 14.985 178.379
4 0.1 g 0.1 1.34
5 0.5 g 0.5 2.868
6 0.2 g 0.2 0.983
7 0.5 g 0.5 9.338
8 0.5 g 0.5 2.08
9 0.5 g 0.5 2.013
10 0.1 g 0.1 -
11 0.5 g 0.5 6.089
12 Trace element solution SL-10 1.0 ml - -
13 20.0 µg 2.0e-5 1.3e-5
14 1000.0 ml 997.21 55353.5
Instructions:
  1. Final pH 9.0 - 9.5
  2. Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
NaCl 49.95 854.687
NaHCO3 14.985 178.379
Na2CO3 4.995 47.128
NH4Cl 0.5 9.338
Show more

Cultivation metadata from 1 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
halophily slightly 1 strain
pH 10 - 14 1 strain
pH 6 - 8 1 strain
Show more

Similar media

1218. ROSEINATRONOBACTER MONICUS MEDIUM 92 %
1389. LAMPROBACTER MEDIUM 89 %
747. DSIC MEDIUM (modified) 87 %
1073. AML60 MEDIUM 87 %
968. ANAEROBACILLUS ARSENICISELENATIS MEDIUM 87 %

Latest comments

No comments for this medium.