Name: ECTOTHIORHODOSPIRA VARIABILIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1224 #

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	50.0	g	50	855.578
Na₂CO₃	5.0	g	5	47.175
NaHCO₃	15.0	g	15	178.557
KCl	0.1	g	0.1	1.341
K₂HPO₄	0.5	g	0.5	2.871
MgCl₂ x 6 H₂O	0.2	g	0.2	0.984
NH₄Cl	0.5	g	0.5	9.348
Na₂S x 9 H₂O	0.5	g	0.5	2.082
Na₂S₂O₃ x 5 H₂O	0.5	g	0.5	2.015
Yeast extract	0.1	g	0.1	-
Na acetate	0.5	g	0.5	6.095
Trace element solution SL-10	1.0	ml	-	-
Vitamin B₁₂	20.0	µg	2.0e-5	1.5e-5
Distilled water	1000.0	ml	-	-
1 Final pH 9.0 - 9.5
2 Prepare and boil the medium in the absence of Na₂S x 9 H₂O, NaHCO₃, Vitamin B₁₂, CaCl₂ x 2 H₂O, MgCl₂ x 6 H₂O. Cool under a stream of nitrogen and add the remaining components, except vitamin B₁₂ and Na₂S2O3 x 5 H₂O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B₁₂ (filter sterilised) and Na₂S2O3 x 5 H₂O from sterile stock solutions.

Trace element solution SL-10 #

1000

Solution occurs in 265 other media (first defined in Medium 320).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 First dissolve FeCl₂ in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

All strains for this medium:

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